期刊
PLANT SCIENCE
卷 304, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2020.110736
关键词
Glycine max; GmAKT1; Salt tolerance; K < sup > plus <; sup > uptake br; Na < sup > plus <; sup >; K < sup > plus <; sup > homeostasis br; K plus uptake; Na; K plus homeostasis
资金
- National Core Soybean Genetic Engineering Project [2016ZX08004002]
The soybean K+ channel GmAKT1 plays a crucial role in K+ uptake and salt stress response. Overexpression of GmAKT1 significantly enhances plant growth and increases K+ concentration, maintaining Na+/K+ homeostasis.
Plant roots absorb K+ from soil via K+ channels and transporters, which are important for stress responses. In this research, GmAKT1, an AKT1-type K+ channel, was isolated and characterized. The expression of GmAKT1 was induced by K+-starvation and salinity stresses, and it was preferentially expressed in the soybean roots. And GmAKT1 was located in the plasma membrane. As an inward K+ channel, GmAKT1 participated in K+ uptake, as well as rescued the low-K+-sensitive phenotype of the yeast mutant and Arabidopsis akt1 mutant. Overexpression of GmAKT1 significantly improved the growth of plants and increased K+ concentration, leading to lower Na+/ K+ ratios in transgenic Arabidopsis and chimeric soybean plants with transgenic hairy roots. In addition, GmAKT1 overexpression resulted in significant upregulation of these ion uptake-related genes, including GmSKOR, GmsSOS1, GmHKT1, and GmNHX1. Our findings suggested that GmAKT1 plays an important part in K+ uptake under low-K+ condition, and could maintain Na+/K+ homeostasis under salt stress in Arabidopsis and soybean plants.
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