Metabolome profiling reveals impact of water limitation on grain filling in contrasting rice genotypes

Drought significantly decreases crop productivity, especially in high water consuming crops like rice. Grain filling is one of the important critical growth phases in rice and drought during this phase leads to significant reduction in yield. In this study, a comparison was made between IR64 (drought susceptible) and Apo (drought tolerant) rice genotypes to capture the response to water limitation (50% field capacity (FC)) compared with the control (100%FC) during grain filling. Plants were grown in a high-throughput phenomics facility for precise imposition of moisture stress during grain filling. Apo performed better in water limited conditions with lower reduction of photosynthetic rate and maintenance of lower leaf temperature than IR64. Days from sowing to maturity, spikelet fertility and seed weight were more impeded by water limitation in IR64 than in Apo. Unlike Apo, IR64 did not show any decrease in transpiration rate at 50%FC compared with 100%FC. Metabolomic profiling of spikelets at grain filling showed distinct effects of water limitation on accumulation of metabolites, especially in Apo. Secondary metabolism, mainly the phenylpropanoid pathway involved in scavenging mechanisms, was upregulated in Apo. Accumulation of most amino acids was significantly higher in IR64 than in Apo. Due to higher rates of photosynthesis under stress, most carbohydrates accumulated more in Apo than in IR64 at 50%FC. Sucrose transporters were significantly upregulated in water limited conditions especially in Apo. Overall, thanks to higher source capacity, more source to sink transport and better scavenging, Apo showed a lower reduction in yield than IR64.

Automated summary

This study compared the response of drought-susceptible IR64 and drought-tolerant Apo rice genotypes under water limitation during grain filling. Apo showed better performance with lower reduction in photosynthetic rate and leaf temperature, as well as less impact on days to maturity, spikelet fertility, seed weight, and transpiration rate compared to IR64. Metabolomic profiling revealed distinct effects of water limitation on metabolite accumulation, with Apo showing upregulation of scavenging mechanisms and higher accumulation of amino acids, while IR64 had higher levels of carbohydrates. Overall, Apo exhibited a lower reduction in yield due to higher source capacity, better source to sink transport, and enhanced scavenging mechanisms.