期刊
PLANT CELL AND ENVIRONMENT
卷 44, 期 5, 页码 1596-1610出版社
WILEY
DOI: 10.1111/pce.14019
关键词
nitrate reductase; nitrite reductase; plant– microbe interaction; Pseudomonas syringae; Ralstonia solanacearum; salicylic acid; Solanum lycopersicum
资金
- Key Research and Development Program of Zhejiang Province [2021C02040]
- Cooperative Extension Plan of Major Agricultural Technologies of Zhejiang Province [2019XTTGSC04]
- Natural Science Foundation of Zhejiang Province [LR19C150001]
- Fundamental Research Funds for the Central Universities
- National Natural Science Foundation of China [31772355, 31822046]
Nitrogen levels influence tomato plant immunity against pathogens, with low nitrogen conditions and NO3- as the sole nitrogen source enhancing resistance. Modulating nitrogen metabolism genes can alter disease susceptibility, with the defense mechanism dependent on the salicylic acid pathway.
Nitrogen (N) influences a myriad of physiological processes while its effects on plant defences and the underlying mechanisms are largely unknown. Here, the interaction between tomato and pathogens was examined under four N regimes (sole NO3- or mixed NO3-/NH4+ of total 1 and 7 mM N, denoting low and high N regimes, respectively) followed by inoculation with two bacterial pathogens, Pseudomonas syringae and Ralstonia solanacearum. Tomato immunity against both pathogens was generally higher under low N as well as NO3- as the sole N source. The disease susceptibility was reduced by silencing N metabolism genes such as NR, NiR and Fd-GOGAT, while increased in NiR1-overexpressed plants. Further studies demonstrated that the N-modulated defence was dependent on the salicylic acid (SA) defence pathway. Low N as well as the silencing of N metabolism genes increased the SA levels and transcripts of its maker genes, and low N-enhanced defence was blocked in NahG transgenic tomato plants that do not accumulate SA, while exogenous SA application attenuated the susceptibility of OE-NiR1. The study provides insights into the mechanisms of how nitrogen fertilization and metabolism affect plant immunity in tomato, which might be useful for designing effective agronomic strategies for the management of N supply.
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