Halothermal and hydrothermal time models describe germination responses of canola seeds to ageing

Hydrothermal time (HTT) and halothermal time (HaloTT) models were used to quantitatively characterize the combined effects of temperature (T), water potential (psi) and NaCl concentration on seed germination of canola after different accelerated ageing periods (AAP) at 42 degrees C and 100% humidity. Seed germination time courses were observed and electrical conductivities of seed leachates were measured in all experiments. The cardinal temperatures estimated by both models were 4.6, 28 and 35 degrees C for the base (T-b), optimum (T-o) and ceiling (T-c) temperatures in water (0 MPa), respectively, when the seeds were not aged. The T-b increased with AAP, while the T-c decreased and the T-o remained constant at all AAP. Below T-o, the median base water potentials (psi(b)(50)) were -1.07, -0.73, -0.48 and -0.39 MPa for the AAP levels of 0, 24, 36 and 48 h, respectively. These values were more negative when germination occurred in salt solutions (the base NaCl concentrations converted to psi were -1.25, -0.81, -0.51 and -0.41 MPa, respectively), due to uptake of salt ions. Thresholds became more positive above T-o and reached zero at T-c in all AAP. Seed osmotic adjustment capacity declined linearly with increasing AAP while the conductivity of seed leachates increased in association with the loss of seed vigour. Significant correlations between conductivity results and the parameters of HTT and HaloTT models suggest that they could be considered as effective descriptors of canola seed vigour.

Automated summary

Hydrothermal time (HTT) and halothermal time (HaloTT) models were utilized to quantitatively analyze the impact of temperature, water potential, and NaCl concentration on canola seed germination. It was found that the base, optimum, and ceiling temperatures were 4.6, 28, and 35 degrees Celsius, respectively, with ageing affecting these temperatures.