Guaiane-type sesquiterpenoids from the roots of Stellera chamaejasme L. and their neuroprotective activities

Nine undescribed guaiane-type sesquiterpenoids stelleraterpenoids A-I, along with seven reported congeners, were isolated and identified from the 70% EtOH extract of the roots of Stellera chamaejasme L. Their chemical structures were elucidated on the basis of various spectral data. The relative configurations were determined by their NOESY spectra and comparison between their experimental and calculated NMR data. The absolute configurations were established by the comparison between the experimental and calculated ECD spectra and further by X-ray single-crystal diffraction analysis. The neuroprotective effects of these compounds on the H2O2-induced damage in human neuroblastoma SH-SY5Y cells were evaluated. Stelleraguaianone B exhibited the better activity with 71.62% cell viability compared to the positive control Trolox (65.05%) at 12.5 mu M, which might be achieved by inhibiting the apoptosis of SH-SY5Y cells based on an annexin V-FITC/PI staining experiment.

Automated summary

Nine undescribed guaiane-type sesquiterpenoids, known as stelleraterpenoids A-I, were isolated from the roots of Stellera chamaejasme L. along with seven reported congeners. The compounds showed neuroprotective effects on H2O2-induced damage in human neuroblastoma SH-SY5Y cells. Stelleraguaianone B exhibited the best activity in inhibiting cell apoptosis compared to the positive control Trolox.