期刊
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
卷 38, 期 4, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ppsc.202100013
关键词
bovine serum albumin; copper sulfide nanoparticles; heat tolerance; mitochondria‐ targeted; photothermal therapy
资金
- HiLIFE Research Funds
- Sigrid Juselius Foundation
- European Research Council Proof-of-Concept Grant [825020]
- National Natural Science Foundation of China [30930077, 31000164]
- Natural Science Foundation of Jiangsu Province [BK20130964]
- Bilateral Chinese-Croatian Scientific Project [6-5]
- European Research Council (ERC) [825020] Funding Source: European Research Council (ERC)
In this study, mitochondria-targeted and highly efficient photothermal R-BSA@CuS nanocomposites were successfully prepared, showing significant anticancer effects on breast cancer cells and the ability to penetrate 3D multicellular tumor spheroids. These nanocomposites have important potential applications in cancer photothermal therapy.
Recently, mitochondria-targeted photothermal nanoagents demonstrated an improved therapeutic efficacy of cancer cells, compared with non-targeting ones. Herein, copper sulfide (CuS) nanoparticles are in situ synthesized via bovine serum albumin (BSA) templates to prepare photothermal BSA@CuS nanocomposites with high efficiency (42.0%) of photothermal conversion. Subsequently, rhodamine-110 (R) molecules are covalently conjugated with BSA@CuS nanocomposites to construct mitochondria-targeted R-BSA@CuS nanocomposites, which still retained 22.8% of photothermal conversion efficiency. Furthermore, as-prepared R-BSA@CuS nanocomposites can be efficiently internalized by human breast cancer (MCF-7) cells, and then specifically accumulated in their subcellular mitochondria, not lysosomes. Compared with non-targeting BSA@CuS nanocomposites, these mitochondria-targeted R-BSA@CuS nanocomposites show a significant enhancement (***p < 0.001) of their anticancer efficacy under the same near-infrared irradiation conditions, whose mechanism is further explored in details. Finally, these R-BSA@CuS nanocomposites can succeed in penetrating in 3D multicellular tumor spheroids composed of MCF-7 cells. And they also show a significant inhibition effect (**p < 0.01) on the growth of spheroids via photothermal therapy, in contrast to bare BSA@CuS nanocomposites under the same irradiation conditions. Therefore, these mitochondria-targeted and photothermal R-BSA@CuS nanocomposites have important potential applications on cancer photothermal therapy with an enhanced efficacy.
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