期刊
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
卷 2021, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2021/8884922
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资金
- National Key R&D Program of China [2018YFB1105700]
- National Natural Science Foundation of China [81772401, 82072505]
- Fundamental Research Funds for the Central Universities [2019kfyXMBZ063]
- Application Foundation and Advanced Program of Wuhan Science and Technology Bureau [2019020701011457]
The study reveals the role of Piezo1 in human NP cells under ECM stiffness, leading to oxidative stress and ER stress, and exacerbating cellular senescence and apoptosis. Inhibition of Piezo1 can alleviate these effects, and silencing Piezo1 may ameliorate IDD and decrease the elasticity modulus of rat NP tissues.
Mechanical stimulation plays a crucial part in the development of intervertebral disc degeneration (IDD). Extracellular matrix (ECM) stiffness, which is a crucial mechanical microenvironment of the nucleus pulposus (NP) tissue, contributes to the pathogenesis of IDD. The mechanosensitive ion channel Piezo1 mediates mechanical transduction. This study purposed to investigate the function of Piezo1 in human NP cells under ECM stiffness. The expression of Piezo1 and the ECM elasticity modulus increased in degenerative NP tissues. Stiff ECM activated the Piezo1 channel and increased intracellular Ca2+ levels. Moreover, the activation of Piezo1 increased intracellular reactive oxygen species (ROS) levels and the expression of GRP78 and CHOP, which contribute to oxidative stress and endoplasmic reticulum (ER) stress. Furthermore, stiff ECM aggravated oxidative stress-induced senescence and apoptosis in human NP cells. Piezo1 inhibition alleviated oxidative stress-induced senescence and apoptosis, caused by the increase in ECM stiffness. Finally, Piezo1 silencing ameliorated IDD in an in vivo rat model and decreased the elasticity modulus of rat NP tissues. In conclusion, we identified the mechanosensitive ion channel Piezo1 in human NP cells as a mechanical transduction mediator for stiff ECM stimulation. Our results provide novel insights into the mechanism of mechanical transduction in NP cells, with potential for treating IDD.
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