Angular spectrum matching for digital holographic microscopy under extremely low light conditions

Digital holographic microscopy (DHM) is a future three-dimensional (3D) microscopy due to its high-resolution and high-precision 3D images. Thus, it is getting attention in bioinformatics, semiconductor defect detection, etc. However, some limitations still exist. Especially, high-speed holographic imaging requires high-power lasers, which are difficult to image on highly absorbent or light-sensitive samples. To overcome these issues, we propose a new, to the best of our knowledge, digital hologram recovery algorithm called angular spectrum matching (ASM), which achieves hologram imitation to recover holograms in digital holography at low light intensities. The hologram used for the background phase comparison is recorded without objects; thus, no power limitation is required. The ASM utilizes this background hologram to recover dark holograms. We present experimental results showing improved DHM numerical reconstructions and recovered holograms under extremely low light conditions. (C) 2021 Optical Society of America

Automated summary

Digital holographic microscopy is a promising technique for high-resolution 3D imaging, but faces challenges with high-power lasers and light-sensitive samples. A new hologram recovery algorithm called angular spectrum matching is proposed to recover holograms at low light intensities, improving digital holographic microscopy imaging.