期刊
NUCLEIC ACIDS RESEARCH
卷 49, 期 6, 页码 3354-3370出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab114
关键词
-
资金
- Russian Science Foundation [19-74-10008, 19-15-00054]
- ERC CZ [LL1601]
- European Regional Developmental Funds [OPVVV16 019/0000759]
- University of Ostrava [SGS/2020]
- American Heart Association [16SDG26420019]
- Russian Science Foundation [19-15-00054, 19-74-10008] Funding Source: Russian Science Foundation
Uridine insertion/deletion (U-indel) editing of mitochondrial mRNA, unique to the protistan class Kinetoplastea, generates canonical as well as potentially non-productive editing events. Analysis reveals that a multitude of editing events are guided by a limited set of gRNAs, with each gRNA possessing the inherent ability to guide both canonical and non-canonical editing.
Uridine insertion/deletion (U-indel) editing of mitochondrial mRNA, unique to the protistan class Kinetoplastea, generates canonical as well as potentially non-productive editing events. While the molecular machinery and the role of the guide (g) RNAs that provide required information for U-indel editing are well understood, little is known about the forces underlying its apparently error-prone nature. Analysis of a gRNA:mRNA pair allows the dissection of editing events in a given position of a given mitochondrial transcript. A complete gRNA dataset, paired with a fully characterized mRNA population that includes non-canonically edited transcripts, would allow such an analysis to be performed globally across the mitochondrial transcriptome. To achieve this, we have assembled 67 minicircles of the insect parasite Leptomonas pyrrhocoris, with each minicircle typically encoding one gRNA located in one of two similar-sized units of different origin. From this relatively narrow set of annotated gRNAs, we have dissected all identified mitochondrial editing events in L. pyrrhocoris, the strains of which dramatically differ in the abundance of individual minicircle classes. Our results support a model in which a multitude of editing events are driven by a limited set of gRNAs, with individual gRNAs possessing an inherent ability to guide canonical and non-canonical editing.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据