期刊
NEW PHYTOLOGIST
卷 230, 期 6, 页码 2355-2370出版社
WILEY
DOI: 10.1111/nph.17323
关键词
genetic improvement; maize; metabolomics profiling; mGWAS; salt tolerance
资金
- Beijing Outstanding Young Scientist Program [BJJWZYJH01201910019026]
- National Natural Science Foundation of China [32071933]
- National Key R&D Program of China [2018YFA0901000]
Research has shown that natural maize varieties display a large diversity of salt tolerance, yet the genetic variants underlying such diversity remain poorly discovered and applied. An LC-MS-based untargeted metabolomics approach identified 37 metabolite biomarkers related to SIOS tolerance, and 10 candidate genes significantly associated with SIOS tolerance and METO abundances. A citrate synthase, a glucosyltransferase, and a cytochrome P450 were validated to underlie genotype-METO-SIOS tolerance associations and improve the SIOS tolerance of elite maize inbred lines through additive effects.
The farmland of the world's main corn-producing area is increasingly affected by salt stress. Therefore, the breeding of salt-tolerant cultivars is necessary for the long-term sustainability of global corn production. Previous studies have shown that natural maize varieties display a large diversity of salt tolerance, yet the genetic variants underlying such diversity remain poorly discovered and applied, especially those mediating the tolerance to salt-induced osmotic stress (SIOS). Here we report a metabolomics-driven understanding and genetic improvement of maize SIOS tolerance. Using a LC-MS-based untargeted metabolomics approach, we profiled the metabolomes of 266 maize inbred lines under control and salt conditions, and then identified 37 metabolite biomarkers of SIOS tolerance (METO1-37). Follow-up metabolic GWAS (mGWAS) and genotype-to-phenotype modeling identified 10 candidate genes significantly associating with the SIOS tolerance and METO abundances. Furthermore, we validated that a citrate synthase, a glucosyltransferase and a cytochrome P450 underlie the genotype-METO-SIOS tolerance associations, and showed that their favorable alleles additively improve the SIOS tolerance of elite maize inbred lines. Our study provides a novel insight into the natural variation of maize SIOS tolerance, which boosts the genetic improvement of maize salt tolerance, and demonstrates a metabolomics-based approach for mining crop genes associated with this complex agronomic trait.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据