4.5 Article

Chronic Ethanol Exposure Potentiates Cholinergic Neurotransmission in the Basolateral Amygdala

期刊

NEUROSCIENCE
卷 455, 期 -, 页码 165-176

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PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2020.12.014

关键词

patch-clamp electrophysiology; presynaptic; withdrawal; acetylcholine; basolateral amygdala

资金

  1. NIH/NIAAA [T32 AA007565, R01 AA023999, R01 AA014445, R21 AA026572, P50 AA026117]

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The study demonstrates that chronic intermittent ethanol exposure can modulate GABA and glutamate inputs in the amygdala through changes in acetylcholine levels, potentially leading to anxiety symptoms.
Chronic intermittent ethanol (CIE) exposure dysregulates glutamatergic and GABAergic neurotransmission, facilitating basolateral amygdala (BLA) pyramidal neuron hyperexcitability and the expression of anxiety during withdrawal. It is unknown whether ethanol-induced alterations in nucleus basalis magnocellularis (NBM) cholinergic projections to the BLA mediate anxiety-related behaviors through direct modulation of GABA and glutamate afferents. Following 10 days of CIE exposure and 24 h of withdrawal, we recorded GABAergic and glutamatergic synaptic responses in BLA pyramidal neurons with electrophysiology, assessed total protein expression of cholinergic markers, and quantified acetylcholine and choline concentrations using a colorimetric assay. We measured alpha(7) nicotinic acetylcholine receptor (nAChR) dependent modulation of presynaptic function at distinct inputs in AIRand CIE-exposed BLA coronal slices as a functional read-out of cholinergic neurotransmission. CIE/withdrawal upregulates the endogenous activity of alpha(7) nAChRs, facilitating release at both GABAergic' local' interneuron and glutamatergic synaptic responses to stria terminalis (ST) stimulation, with no effect at GABAergic lateral paracapsular cells (LPCs). CIE caused a three-fold increase in BLA acetylcholine concentration, with no changes in alpha(7) nAChR or cholinergic marker expression. These data illustrate that alpha(7) nAChRdependent changes in presynaptic function serve as a proxy for CIE-dependent alterations in synaptic acetylcholine levels. Thus, cholinergic projections appear to mediate CIE-induced alterations at GABA/glutamate inputs. (c) 2020 IBRO. Published by Elsevier Ltd. All rights reserved.

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