期刊
NEUROPHARMACOLOGY
卷 183, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuropharm.2020.108394
关键词
OTR; 5-HTR2C; Heteroreceptor complexes; GPCR crosstalk; Hypoactivity
资金
- Science Foundation Ireland Research Centre [SFI/12/RC/2273]
- National Science Centre, Poland [2019/03/X/NZ1/00607]
- Swedish Medical Research Council [62X-00715-50-3]
- Hjarnfonden [F02018-0286]
- Karolinska Institutet Forskningsstiftelser
- Olle Engkvist Stiftelse
This article explores the signaling interaction between oxytocin and serotonin, particularly focusing on the potential physical interaction between oxytocin receptor (OTR) and 5-hydroxytryptamine 2C receptor (5-HTR2C) and the formation of functionally active OTR/5-HTR2C heterocomplexes. The study provides evidence for the involvement of these heterocomplexes in the regulation of downstream signaling pathways and behaviors, including OT-mediated hypoactivity in mice enhanced by 5-HTR2C antagonism.
The complexity of oxytocin-mediated functions is strongly associated with its modulatory effects on other neurotransmission systems, including the serotonin (5-hydroxytryptamine, 5-HT) system. Signalling between oxytocin (OT) and 5-HT has been demonstrated during neurodevelopment and in the regulation of specific emotion-based behaviours. It is suggested that crosstalk between neurotransmitters is driven by interaction between their specific receptors, particularly the oxytocin receptor (OTR) and the 5-hydroxytryptamine 2C receptor (5-HTR2C), but evidence for this and the downstream signalling consequences that follow are lacking. Considering the overlapping central expression profiles and shared involvement of OTR and 5-HTR2C in certain endocrine functions and behaviours, including eating behaviour, social interaction and locomotor activity, we investigated the existence of functionally active OTR/5-HTR2C heterocomplexes. Here, we demonstrate evidence for a potential physical interaction between OTR and 5-HTR2C in vitro in a cellular expression system using flow cytometry-based FRET (fcFRET). We could recapitulate this finding under endogenous expression levels of both receptors via in silico analysis of single cell transcriptomic data and ex vivo proximity ligation assay (PLA). Next, we show that co-expression of the OTR/5-HTR2C pair resulted in a significant depletion of OTR-mediated G alpha q-signalling and significant changes in receptor trafficking. Of note, attenuation of OTR-mediated downstream signalling was restored following pharmacological blockade of the 5-HTR2C. Finally, we demonstrated a functional relevance of this novel heterocomplex, in vivo, as 5-HTR2C antagonism increased OT-mediated hypoactivity in mice. Overall, we provide compelling evidence for the formation of functionally active OTR/5-HTR2C heterocomplexes, adding another level of complexity to OTR and 5-HTR2C signalling functionality. This article is part of the special issue on Neuropeptides.
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