期刊
ENGINEERING IN LIFE SCIENCES
卷 16, 期 5, 页码 424-431出版社
WILEY
DOI: 10.1002/elsc.201500161
关键词
Citric acid; Dissolved oxygen; Oleic acid; Optimization; Oxygen-vector
资金
- National Natural Science Foundation of China [21406083]
- Natural Science Foundation of Jiangsu Province [BK20150412]
- Natural Science Fund for Colleges and Universities in Jiangsu Province [13KJB480001, 15KJD180006]
- Huaian Municipal Science and Technology Support Program of China [HAG2014029, HAG2014032]
Yarrowia lipolytica is able to secrete large amounts of citric acid (CA), which is greatly affected by the dissolved oxygen concentration (DOC) in the fermentation medium. In this study, oleic acid was selected as oxygen-vector to improve DOC during CA fermentation. When 2% (v/v) of oleic acid was added to the culture broth, higher DOC (>42.1%) was determined throughout the CA synthesis phase. The yield of CA reached a maximum of 32.1 g/L (25.4% higher than the control) and the biomass was 8.8 g/L. The substrate uptake rate, products formation rate and key enzyme activities were also determined, and the results indicated that CA synthesis was strengthened with oleic acid addition. Furthermore, it was detected that oleic acid could be assimilated by the cells, which means that oleic acid could be served both as oxygen-vector and co-substrate for CA synthesis by Y. lipolytica. In a bioreactor with working volume of 3 L, the highest concentration of CA reached to 36. 4 g/L in the presence of 2% (v/v) oleic acid after 192 h of fermentation. These results confirmed that oleic acid could be applied in the large-scale production of CA by Y. lipolytica.
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