Identification, isolation and analysis of human gut-associated lymphoid tissues

The authors present a protocol for isolating and studying human gut-associated lymphoid tissue. Gut-associated lymphoid tissues (GALTs) comprise key intestinal immune inductive sites, including the Peyer's patches of the small intestine and different types of isolated lymphoid follicle (ILF) found along the length of the gut. Our understanding of human GALT is limited due to a lack of protocols for their isolation. Here we describe a technique that, uniquely among intestinal cell isolation protocols, allows identification and isolation of all human GALT, as well as GALT-free intestinal lamina propria (LP). The technique involves the mechanical separation of intestinal mucosa from the submucosa, allowing the identification and isolation of submucosal ILF (SM-ILF), LP-embedded mucosal ILF (M-ILF) and LP free of contaminating lymphoid tissue. Individual SM-ILF, M-ILF and Peyer's patch follicles can be subsequently digested for downstream cellular and molecular characterization. The technique, which takes 4-10 h, will be useful for researchers interested in intestinal immune development and function in health and disease.

Automated summary

The authors present a protocol for isolating and studying human gut-associated lymphoid tissue, which allows identification and isolation of all human GALT and GALT-free intestinal lamina propria. This technique involves mechanical separation of intestinal mucosa from the submucosa to identify and isolate specific types of lymphoid tissue for downstream cellular and molecular characterization. The protocol, taking 4-10 hours, could be useful for researchers interested in intestinal immune development and function in health and disease.