期刊
NATURE METHODS
卷 18, 期 3, 页码 303-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41592-020-01052-9
关键词
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资金
- National Institute of General Medical Sciences [P41 GM108569]
- NIH [S10OD025194, RF1AG063903, R44GM116584, R44CA212733, R44CA214076]
- Thermo Fisher Scientific
- Chemistry of Life Processes Predoctoral Training grant at Northwestern University [T32GM105538]
- Transition to Independence grant [K99CA234434-01]
Nuc-MS effectively displays information about histone variants and their post-translational modifications, showing high concordance with ChIP-seq results and providing a new readout of nucleosome-level biology.
Current proteomic approaches disassemble and digest nucleosome particles, blurring readouts of the 'histone code'. To preserve nucleosome-level information, we developed Nuc-MS, which displays the landscape of histone variants and their post-translational modifications (PTMs) in a single mass spectrum. Combined with immunoprecipitation, Nuc-MS quantified nucleosome co-occupancy of histone H3.3 with variant H2A.Z (sixfold over bulk) and the co-occurrence of oncogenic H3.3K27M with euchromatic marks (for example, a >15-fold enrichment of dimethylated H3K79me2). Nuc-MS is highly concordant with chromatin immunoprecipitation-sequencing (ChIP-seq) and offers a new readout of nucleosome-level biology.
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