Imaging-based screens of pool-synthesized cell libraries

Mapping a genetic perturbation to a change in phenotype is at the core of biological research. Advances in microscopy have transformed these studies, but they have largely been confined to examining a few strains or cell lines at a time. In parallel, there has been a revolution in creating synthetic libraries of genetically altered cells with relative ease. Here we describe methods that combine these powerful tools to perform live-cell imaging of pool-generated strain libraries for improved biological discovery.

Automated summary

Linking genetic perturbations to changes in phenotype is crucial in biological research. Advances in microscopy and the creation of genetically altered cell libraries have allowed for live-cell imaging of pool-generated strain libraries, enhancing biological discovery.