4.8 Article

Cell competition constitutes a barrier for interspecies chimerism

期刊

NATURE
卷 592, 期 7853, 页码 272-+

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NATURE PORTFOLIO
DOI: 10.1038/s41586-021-03273-0

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资金

  1. Cancer Prevention & Research Institute of Texas [CPRIT RR170076]
  2. Hamon Center for Regenerative Science Medicine
  3. National Science Foundation [2019241092]
  4. NIH [R01 AR053173, R01 GM098816]
  5. HHMI Faculty Scholar Award
  6. Guangdong Provincial Key Laboratory of Genome Read and Write [2017B030301011]

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Cell competition is a fitness-sensing process where fitter cells eliminate less-fit but viable cells. The study developed an interspecies PSC co-culture strategy and identified a previously unknown mode of cell competition between species. Genetic inactivation of key components of the NF-kappa B complex in human cells improved the survival and chimerism of human cells in early mouse embryos.
Cell competition involves a conserved fitness-sensing process during which fitter cells eliminate neighbouring less-fit but viable cells(1). Cell competition has been proposed as a surveillance mechanism to ensure normal development and tissue homeostasis, and has also been suggested to act as a barrier to interspecies chimerism(2). However, cell competition has not been studied in an interspecies context during early development owing to the lack of an in vitro model. Here we developed an interspecies pluripotent stem cell (PSC) co-culture strategy and uncovered a previously unknown mode of cell competition between species. Interspecies competition between PSCs occurred in primed but not naive pluripotent cells, and between evolutionarily distant species. By comparative transcriptome analysis, we found that genes related to the NF-kappa B signalling pathway, among others, were upregulated in less-fit 'loser' human cells. Genetic inactivation of a core component (P65, also known as RELA) and an upstream regulator (MYD88) of the NF-kappa B complex in human cells could overcome the competition between human and mouse PSCs, thereby improving the survival and chimerism of human cells in early mouse embryos. These insights into cell competition pave the way for the study of evolutionarily conserved mechanisms that underlie competitive cell interactions during early mammalian development. Suppression of interspecies PSC competition may facilitate the generation of human tissues in animals.

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