4.4 Article

Assessment of antiviral activity and mechanism of rhein on newcastle disease virus (La Sota strain IV) in vitro

期刊

NATURAL PRODUCT RESEARCH
卷 36, 期 5, 页码 1400-1404

出版社

TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2021.1878515

关键词

Chicken embryo fibroblasts; newcastle disease; traditional Chinese medicine; rhein

资金

  1. Science and Technology Support Program of Sichuan Province [2015SZ0201, 20ZDYF1246]

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This study investigated the antiviral activities of rhein against Newcastle disease virus (NDV) and found that rhein effectively inhibited NDV activities at a maximal safe concentration of 0.125 mg/ml. This suggests rhein could be potentially used as a future antiviral drug against NDV.
Current research is focused on the development of drug candidates from natural products. Rhein a Traditional Chinese Medicine (TCM) from Polygonaceae (rhubarb) has exhibited antioxidant, anti-inflammatory and anticancer activities, however no work has reported its antiviral potential, thus this study was performed to investigate the antiviral activities of rhein on new castle disease virus (NDV) in vitro. NDV infection of chicken embryo fibroblasts (CEFs) was prepared using 10-day-old specific pathogen free chicken embryos. Cytotoxicity and anti-viral activities of rhein were assessed using the MTT method. The interaction between NDV and cell membrane proteins were also detected using virus overlay protein binding assay (VOPBA). In addition NDV genes expressions in CEFs were measured using real-time fluorescent quantitative (RTFQ) PCR. The results showed that rhein effectively inhibit NDV activities maximal safe concentration of 0.125 mg/ml. This finding indicated that, rhein could be used as future antiviral drug against NDV.

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