期刊
NATURAL PRODUCT RESEARCH
卷 36, 期 5, 页码 1396-1399出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2021.1878514
关键词
Ganoderma lucidum; sterol; triterpenoid; multidrug resistance; P-glycoprotein; antitumor
资金
- Natural Science Foundation of Fujian Province of China [2020J01624, 2020J01617]
- National natural science foundation of China [81202560]
- Chinese medicine modernization project of Ministry of science and technology of China [2019YFC1710500]
Various compounds were isolated and identified from Ganoderma lucidum, with ganoderiol F showing potent activity in reversing P-glycoprotein mediated tumor multidrug resistance.
Two sterols and seven triterpenoids were isolated and identified from Ganoderma lucidum by silica gel column chromatography, preparative high-performance liquid chromatography and spectra analysis. Then, the multidrug resistance reversal activities of these compounds were assessed using MTT assay. Among these compounds, ganoderol B (3), ganoderone A (4), ganodermanondiol (6) and ganoderiol F (8) were shown to reverse the resistance of human oral epidermoid carcinoma cell line KBv200 to doxorubicin, and the reversal folds were 6.59, 4.70, 4.01 and 7.09, respectively. Ganoderiol F could increase the intracellular accumulation of doxorubicin in KBv200 cells through inhibiting P-glycoprotein transport function. Further mechanistic investigation found that ganoderiol F did not alter P-glycoprotein expression. In conclusion, ganoderiol F has potent effect in reversing P-glycoprotein mediated tumor multidrug resistance. Potential reversal agents against multidrug resistance in tumor may be found in triterpenoids from Ganoderma lucidum.
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