4.6 Article

Preferential uptake of antibody targeted calcium phosphosilicate nanoparticles by metastatic triple negative breast cancer cells in co-cultures of human metastatic breast cancer cells plus bone osteoblasts

出版社

ELSEVIER
DOI: 10.1016/j.nano.2021.102383

关键词

Metastatic breast cancer; Bone; Osteoblast; Gemcitabine; Nanodelivery; Nanoparticle

资金

  1. National Cancer Institute (NCI), NIH [R01CA167535, R21CA170121, NCI CA178177-05]
  2. Pennsylvania Department of Health, Tobacco CURE funds SAP [4100072562, 4100072566]
  3. Pennsylvania Breast Cancer Coalition
  4. National Center for Advancing Translational Sciences (NCATS) [UL1 TR000127, TL1 TR000125]

向作者/读者索取更多资源

The study demonstrated that targeting anti-CD71-CPSNPs enhanced internalization by triple negative breast cancer cells in vitro, leading to apoptotic responses. CPSNPs may serve as imaging tools and selective drug delivery systems for the treatment of breast cancer metastasized to bone.
Calcium phosphosilicate nanoparticles (CPSNPs) are bioresorbable nanoparticles that can be bioconjugated with targeting molecules and encapsulate active agents and deliver them to tumor cells without causing damage to adjacent healthy tissue. Data obtained in this study demonstrated that an anti-CD71 antibody on CPSNPs targets these nanoparticles and enhances their internalization by triple negative breast cancer cells in-vitro. Caspase 3,7 activation, DNA damage, and fluorescent microscopy confirmed the apoptotic breast cancer response caused by targeted anti-CD71-CPSNPs encapsulated with gemcitabine monophosphate, the active metabolite of the chemotherapeutic gemcitabine used to treat cancers including breast and ovarian. Targeted anti-CD71-CPSNPs encapsulated with the fluorophore, Rhodamine WT, were preferentially internalized by breast cancer cells in co-cultures with osteoblasts. While osteoblasts partially internalized anti-CD71GemMP-CPSNPs, their cell growth was not affected. These results suggest that CPSNPs may be used as imaging tools and selective drug delivery systems for breast cancer that has metastasized to bone. (C) 2021 Elsevier Inc. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据