期刊
NANO LETTERS
卷 21, 期 5, 页码 2224-2231出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.0c05039
关键词
RNA vaccine; immunotherapy; long-term release; graphene oxide; polyethylenimine
类别
资金
- National Key R&D Program of China [2018YFE0205300, 2018YFA0208900]
- NSFC [31971307, 32000950]
- Key Research Project of Frontier Science of the Chinese Academy of Sciences [QYZDJ-SSW-SLH022]
- Innovation Research Group of National Natural Science Foundation [11621505]
- K.C. Wong Education Foundation [GJTD-2018-03]
- Key Laboratory of Biomedical Effects of Nanomaterials and Nanosafety, CAS [NSKF202009]
The research team developed a transformable hydrogel that can generate nanovaccines for up to 30 days after injection, showing promising potential for effective cancer immunotherapy.
Messenger RNA (mRNA) vaccine is a promising candidate in cancer immunotherapy as it can encode tumor-associated antigens with an excellent safety profile. Unfortunately, the inherent instability of RNA and translational efficiency are major limitations of RNA vaccine. Here, we report an injectable hydrogel formed with graphene oxide (GO) and polyethylenimine (PEI), which can generate mRNA (ovalbumin, a model antigen) and adjuvants (R848)-laden nanovaccines for at least 30 days after subcutaneous injection. The released nanovaccines can protect the mRNA from degradation and confer targeted delivering capacity to lymph nodes. The data show that this transformable hydrogel can significantly increase the number of antigen-specific CD8(+) T cells and subsequently inhibit the tumor growth with only one treatment. Meanwhile, this hydrogel can generate an antigen specific antibody in the serum which in turn prevents the occurrence of metastasis. Collectively, these results demonstrate the potential of the PEI-functionalized GO transformable hydrogel for effective cancer immunotherapy.
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