期刊
MOLECULAR AND CELLULAR BIOCHEMISTRY
卷 476, 期 5, 页码 2193-2201出版社
SPRINGER
DOI: 10.1007/s11010-021-04073-w
关键词
MCAO; BMSCs; PI3K; AKT; CRMP-2; GAP-43; Axon growth
类别
资金
- National Natural Science Foundation of China [81772452, 81802225]
- Fujian Provincial Special Foundation for Natural Science Innovation Project [2016B014]
- Joint Funds for the Innovation of Science and Technology, Fujian province [2017Y9030, 2019Y9058]
- Natural Science Foundation of Fujian Province [2020J011017]
- Teacher Education Research Project of Fujian Education Department [JAT170220]
- Fujian Provincial Health Technology Project [2019-ZQN-51]
Transplantation of BMSCs activates the PI3K/AKT signaling pathway to promote neurological function recovery after cerebral ischemia. This is associated with increased NF-200 density and expression of p-AKT, pGSK-3β, and GAP-43, while decreasing pCRMP-2 expression to accelerate neurological deficit recovery.
Background: Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) is a potential therapy for cerebral ischemia. However, the underlying protective mechanism remains undetermined. Here, we tested the hypothesis that transplantation of BMSCs via intravenous injection can alleviate neurological functional deficits through activating PI3K/AKT signaling pathway after cerebral ischemia in rats. Methods: A cerebral ischemic rat model was established by the 2 h middle cerebral artery occlusion (MCAO). Twenty-four hours later, BMSCs (1 x 10(6) in 1 ml PBS) from SD rats were injected into the tail vein. Neurological function was evaluated by modified neurological severity score (mNSS) and modified adhesive removal test before and on d1, d3, d7, d10 and d14 after MCAO. Protein expressions of AKT, GSK-3 beta, CRMP-2 and GAP-43 were detected by Western-bolt. NF-200 was detected by immunofluorescence. Results: BMSCs transplantation did not only significantly improve the mNSS score and the adhesive-removal somatosensory test after MCAO, but also increase the density of NF-200 and the expression of p-AKT, pGSK-3 beta and GAP-43, while decrease the expression of pCRMP-2. Meanwhile, these effects can be suppressed by LY294002, a specific inhibitor of PI3K/AKT. Conclusion: These data suggest that transplantation of BMSCs could promote axon growth and neurological deficit recovery after MCAO, which was associated with activation of PI3K/AKT /GSK-3 beta/CRMP-2 signaling pathway.
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