期刊
MICROCHIMICA ACTA
卷 188, 期 3, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-020-04699-5
关键词
Multi-colorimetric immunoassay; Gold nanobipyramids; Octahedral Cu2O; Local surface plasmon resonance; Ochratoxin A
资金
- National Natural Science Foundation of China [21874048, 21705051]
- Key R&D Program of Guangdong Province [2019B020219003]
- Educational Commission Foundation of Guangdong Province [2020ZDZX2025]
- Open Fund of State Key Laboratory of Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products [2010DS700124-KF1911]
- Program for the Top Young Innovative Talents of Guangdong Province [2016TQ03N305]
A multi-colorimetric immunosensor based on the mimetic enzyme etching of gold nanobipyramids was established to detect ochratoxin A. The method showed high sensitivity and specificity, indicating its potential for OTA detection in real samples.
A multi-colorimetric immunosensor basing on the mimetic enzyme etching of gold nanobipyramids (Au NBPs) was established to detect ochratoxin A (OTA). Octahedral Cu2O nanoparticles were successfully synthesized through a selective surface stabilization strategy, which can exhibit a peroxidase-like ability to oxidize 3,3 ',5,5 '-tetramethylbenzidine (TMB). Au NBPs can be etched by the product, TMB2+, to form a significant longitudinal peak blue shift of local surface plasmon resonance. During the construction of the immunosensor, the microplate was coated with dopamine to immobilized OTA antigens, followed by the immunoreaction of OTA antibody and the Cu2O-labled secondary antibody. A linear relationship can be found between the local surface plasmon resonance (LSPR) peak changes with the logarithm of OTA concentration in a wide range from 1 ng/L to 5 mu g/L, while the detection limit was 0.47 ng/L. Meanwhile, the approximate OTA concentration can be conveniently and intuitively observed by the vivid color changes. Benefiting from the high specificity, the proposed multi-colorimetric immunoassay detection of OTA in millet samples was achieved, indicating the available potential of the immunoassay for the determination of OTA in real samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据