Electrochemical aptasensor of bisphenol A constructed based on 3D mesoporous structural SBA-15-Met with a thin layer of gold nanoparticles

Herein, a simple and sensitive electrochemical aptasensor was fabricated on the electrodeposited nanoporous gold electrode. A layer of gold nanoparticle deposited on the surface of 3D mesoporous structures of SBA-15-Met provided a clean, stable, and biocompatible surface for self-assemble of chain BPA aptamers that bring about considerable advantages in both molecular recognition and signal enhancement. The most important factors affecting the design of the aptasensor, such as aptamer concentration, aptamer incubation time, BPA incubating time, and the pH of the solution, were optimized using a multivariate experiment design. For this purpose, a five level central composite design and a response surface methodology were used. Under the optimized conditions, the proposed aptasensor exhibited a wide linear range from 10 to 1200 pM with an ultralow detection limit of 3.65 pM for BPA. According to molecular docking computational, some hydrogen bonds and the pi-pi interactions have led to a strong affinity between the aptamer and BPA molecules which causes the developed aptasensor to have high sensitivity and selectivity for BPA determination in food samples.

Automated summary

A simple and sensitive electrochemical aptasensor was fabricated on the electrodeposited nanoporous gold electrode, optimizing key factors affecting the design of the aptasensor and demonstrating high sensitivity and selectivity for BPA.