4.7 Article

Community Composition and Co-Occurrence Patterns of Diazotrophs along a Soil Profile in Paddy Fields of Three Soil Types in China

期刊

MICROBIAL ECOLOGY
卷 82, 期 4, 页码 961-970

出版社

SPRINGER
DOI: 10.1007/s00248-021-01716-9

关键词

Soil diazotrophs; Soil profile; nifH; MiSeq sequencing; Co-occurrence network; Paddy soil

资金

  1. Strategic Priority Research Program of the Chinese Academy of Sciences [XDB15010101]
  2. National Natural Science Foundation of China [41671258]

向作者/读者索取更多资源

The research found that diazotrophs in paddy soils mainly belong to the phylum Proteobacteria, with the highest diversity in the 10-20 cm layer and decreasing with soil depth. The community structure of diazotrophs is significantly influenced by region and soil depth, with soil total C and N content and pH being the main factors affecting vertical variation.
Diazotrophs play a key role in biological nitrogen (N-2) fixation. However, we know little about the distribution of the diazotrophic community along the soil profile in paddy fields. Here, we used Illumina MiSeq sequencing, targeting the nitrogenase reductase (nifH) gene, to investigate changes with depth (0-100 cm) in the diazotrophic community in paddy soils of three regions (Changshu, Hailun, and Yingtan) in China. The results indicated that most diazotrophs belonged to the phylum Proteobacteria, accounting for 78.05% of the total number of sequences. The diazotrophic diversity was generally highest in the 10-20 cm layer, and then significantly decreased with soil depth. Principal coordinate analysis and PERMANOVA indicated that the diazotrophic community structure was significantly affected by region and soil depth. There were obvious differences in the composition of the diazotrophic community between the topsoil (0-40 cm) and the subsoil (40-100 cm). Anaeromyxobacter, Sideroxydans, Methylomonas, Nostoc, Methanocella, and Methanosaeta were enriched in the topsoil, while Geobacter, Azoarcus, Bradyrhizobium, and Dechloromonas were concentrated in the subsoil. Furthermore, co-occurrence network analysis showed that the diazotrophic network in the topsoil was more complex than that in the subsoil. Distance-based redundancy analysis indicated that soil total C and N content and pH were the main factors influencing the vertical variation in the diazotrophic community. These results highlighted that depth has a great impact on the diazotrophic diversity, community composition, and co-occurrence patterns in paddy soil.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据