期刊
MICROBIAL DRUG RESISTANCE
卷 27, 期 9, 页码 1271-1281出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/mdr.2020.0346
关键词
heterogeneous vancomycin-intermediate staphylococcus aureus; comparative genomic analysis; two-component systems; genetic mutation
资金
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI14C2658]
Recent genomic studies have shown genetic diversity in methicillin-resistant Staphylococcus aureus (MRSA). In this study, genetic variations and features of heterogeneous vancomycin-intermediate S. aureus (hVISA) strains in South Korea were investigated, revealing different mechanisms for reduced vancomycin susceptibility in each clonal lineage, particularly in two-component systems (TCSs) and transcriptional regulators such as walK. ST5 and ST239 clones were closely associated with the epidemiological features of hVISA strains.
Recent genomic studies of methicillin-resistant Staphylococcus aureus (MRSA) have revealed genetic diversity in the various clonal lineages. Along with clinical concerns of MRSA infection, infection with heterogeneous vancomycin-intermediate S. aureus (hVISA) is closely associated with treatment failure. In this study, we investigated the magnitude of genetic variation and features at the genomic level of hVISA strains isolated in South Korea. Four hVISA strains were analyzed by molecular epidemiology, antimicrobial susceptibility, and whole-genome sequencing methods, and they were compared with the reference VISA and vancomycin-susceptible S. aureus strains in the same clonal lineage. The epidemiologic features of hVISA strains were closely related to the ST5 and ST239 clones. Comparative analysis of the whole genome showed genetic mutations, particularly in two-component systems (TCSs) and transcriptional regulators. Genetic mutations in walK were commonly found in both ST5- (F545L, E378K, T500K) and ST239-related (E424D, T492R) hVISA strains. hVISA strains in the ST5 clonal lineage contained mutations in TCS genes, including the walK, vraR, and agr loci, whereas ST239-related strains harbored different genetic variations in walK, lytR, and saeR. This study suggests that the diverse genetic variation of TCSs and transcriptional regulators are involved in reduced vancomycin susceptibility through different mechanisms in each clonal lineage.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据