Serial propagation in water-in-oil emulsions selects for Saccharomyces cerevisiae strains with a reduced cell size or an increased biomass yield on glucose

In S. cerevisiae and many other micro-organisms an increase in metabolic efficiency (i.e. ATP yield on carbon) is accompanied by a decrease in growth rate. From a fundamental point of view, studying these yield-rate trade-offs provides insight in for example microbial evolution and cellular regulation. From a biotechnological point of view, increasing the ATP yield on carbon might increase the yield of anabolic products. We here aimed to select S. cerevisiae mutants with an increased biomass yield. Serial propagation of individual cells in water-in-oil emulsions previously enabled the selection of lactococci with increased biomass yields, and adapting this protocol for yeast allowed us to enrich an engineered Crabtree-negative S. cerevisiae strain with a high biomass yield on glucose. When we started the selection with an S. cerevisiae deletion collection, serial propagation in emulsion enriched hxk2 Delta and reg1 Delta strains with an increased biomass yield on glucose. Surprisingly, a tps1 Delta strain was highly abundant in both emulsion-and suspension-propagated populations. In a separate experiment we propagated a chemically mutagenized S. cerevisiae population in emulsion, which resulted in mutants with a higher cell number yield on glucose, but no significantly changed biomass yield. Genome analyses indicate that genes involved in glucose repression and cell cycle processes play a role in the selected phenotypes. The repeated identification of mutations in genes involved in glucose-repression indicates that serial propagation in emulsion is a valuable tool to study metabolic efficiency in S. cerevisiae.

Automated summary

Studying the trade-offs between metabolic efficiency and growth rate in microorganisms like S. cerevisiae can provide insights into microbial evolution and cellular regulation, with potential biotechnological applications. Successful enrichment of S. cerevisiae mutants with increased biomass yield was achieved through serial propagation in water-in-oil emulsions. This method was shown to be a valuable tool for studying metabolic efficiency in S. cerevisiae by repeatedly identifying mutations in genes related to glucose repression during the selection process.