期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 144, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2021.111262
关键词
Eugenol; Cationic starch; Phospholipid nanofibers; Antibacterial; Bacillus cereus
资金
- State Key Laboratory of Utilization of Woody Oil Resource [2019XK 2002]
- National Natural Science Foundation of China [31972172]
- Natural Science Foundation of Jiangsu Province [BK20201417]
- Jiangsu Province Research Fund [JNHB-131]
- Jiangsu University Research Fund [11JDG050]
A novel controlled-release antibacterial nanofiber was fabricated by electrospinning, successfully loading eugenol@cationic starch nanoparticles into phospholipid nanofibers to achieve controlled release of eugenol against Bacillus cereus. The nanofibers demonstrated significant antibacterial activity against B. cereus and did not damage the color and texture of beef samples.
A novel controlled-release antibacterial nanofiber was fabricated by electrospinning to load eugenol@cationic starch nanoparticles (EG@CStNPs) into phospholipid nanofibers (EG@CSt/P nanofibers). Taking advantage of the phospholipase and amylase secreted by Bacillus cereus (B. cereus), the controlled release of eugenol in EG@CSt/P nanofibers was achieved through the hydrolysis of the phospholipids in the nanofibers and the cationic starch of the nanoparticles. EG@CStNPs were prepared by nanoprecipitation and characterized by SEM, FTIR, and Raman spectroscopy. The preparation method of phospholipid nanofibers was improved, and nanofibers with enhanced properties were identified through their physical properties. The success of EG@CStNP loading into phospholipid nanofibers was confirmed by FTIR, SEM, AFM and TGA. Subsequently, the success of controlled release was verified by GC-MS, inhibition-zone experiments and TEM. After treatment with EG@CSt/P nanofibers for 5 days, the number of B. cereus decreased by around 2 log(10) reductions at 4 degrees C and 25 degrees C. The results of experiments performed for application in beef further showed that EG@CSt/P nanofibers had significant antibacterial activity against B. cereus and did not damage the color and texture of beef samples.
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