期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 138, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.lwt.2020.110680
关键词
Postharvest ripening; Amino acids; SEC-MS/MS; Calcium carbide; Principal component analysis
资金
- Food Safety and Standard Authority of India [73/R&D/Artificial Ripeners/RARD/2016-FSSAI, GAP-0602]
- Council of Scientific and Industrial Research (CSIR)
A novel analytical method was developed for the quantification of free amino acids in mango fruit pulp, showing significant changes in amino acid profiles under different ripening agents. The principal component analysis indicated that fruits ripened with calcium carbide are similar to naturally ripened fruits.
A simple and sensitive size exclusion chromatography - liquid chromatography tandem mass spectrometry (SEC-MS/MS) based analytical method was developed for the quantification of free amino acids from mango fruit pulp samples. The pulp samples were extracted with 70:30 acetonitrile:water containing 0.05 mol/L formic acid and treated with activated charcoal to remove the phenolic and hydrophobic impurities. The amino acids were isolated by strong cation exchange process and quantified by SEC-MS/MS. The method was validated for linearity, the limit of detection and limit of quantification. The LOQ values were observed in the range of 0.2-145 ng/g at 10:1 signal to noise ratio and the interday and intraday precisions were below 15%. The developed method was applied for the analysis of amino acids in mango fruits ripened with ethylene, calcium carbide and ethephon ripening agents. Alanine was observed as major amino acid and other amino acids showed significant changes, which were studied by ANOVA and principal component analysis (PCA). The PCA studies showed that the fruits ripened with calcium carbide are similar to that of naturally ripened fruits.
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