期刊
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
卷 33, 期 3, 页码 591-594出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1040638721996685
关键词
canine; cerebrospinal fluid; genotype; toxoplasmosis; virulence
资金
- Bahia Research Foundation (FAPESB) [PNE0003/2011]
- Coordination for the Improvement of Higher Education Personnel (CAPES)
- National Council for Scientific and Technological Development (CNPq)
The study identified parasites resembling Neospora caninum or Toxoplasma gondii in the cerebrospinal fluid of a dog with neurologic disease, which had not been previously reported in dogs. The detection of T. gondii by CSF cytology was crucial for diagnosing toxoplasmosis in the examined dog.
Parasites resembling Neospora caninum or Toxoplasma gondii were detected by cytologic examination of cerebrospinal fluid (CSF) from a dog with neurologic disease. The dog became severely ill and was euthanized. Canine tissue homogenates were used for direct parasite isolation in cell culture, bioassay in 2 mouse lineages, and PCR. T. gondii was isolated in monkey kidney cells, and species identity was confirmed by PCR. Inoculated parasites were highly virulent for mice, which developed clinical signs and were euthanized immediately. PCR-RFLP for T. gondii using the cultured isolate (TgDgBA22) was conducted with 12 genetic markers, and a unique recombinant strain was identified. Detection of T. gondii by CSF cytology, although described in humans, had not been reported previously in dogs, to our knowledge, and was crucial for the diagnosis of toxoplasmosis in the examined dog.
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