期刊
JOURNAL OF SEPARATION SCIENCE
卷 44, 期 9, 页码 1913-1922出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202001230
关键词
alkylresorcinol; countercurrent chromatography; GC; MS; rye
资金
- Projekt DEAL
This study isolated high-purity alkylresorcinols from rye using countercurrent chromatography, providing standard materials for subsequent bioassays.
Alkylresorcinols (5-alkyl-1,3-dihydroxybenzenes) are amphiphilic phenolic lipid compounds that are abundant in cereals with highest contents in rye. Alkylresorcinols are suspected to show a wide range of favourable biological activities. For such and further testing, highly pure alkylresorcinol standards are required. Especially, purities >> 98% were partly difficult to obtain in the past. Here, we aimed to isolate the most abundant (saturated) alkylresorcinols from rye using countercurrent chromatography. To achieve very high purity, alkylresorcinol-containing extract (similar to 7.14 g) of rye grains (cold extracts with cyclohexane/ethyl acetate (46/54, w/w)) were preparatively transesterified followed by a preparative hydrogenation. Countercurrent chromatography separation of similar to 1 g hydrogenated and transesterified rye grain extract using the solvent system n-hexane-ethyl acetate-methanol-water (9:1:9:1, v/v/v/v) yielded 51.8 mg AR17:0, 77.4 mg AR19:0, 57.2 mg AR21:0, 28.8 mg AR23:0 and 11.5 mg AR25:0 with purities >99% in either case. The isolated alkylresorcinol homologues can be used for subsequent bioassays.
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