期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 350, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jneumeth.2020.109043
关键词
Collagen 1; Electrospinning; Guidance cue; Hydrogel; Polylactide; Tissue engineering
资金
- Academy of Finland, Finland [286990, 326436, 312414, 311017]
- Instrumentarium Science Foundation [200011]
- Academy of Finland (AKA) [311017, 312414, 286990, 311017, 312414, 286990] Funding Source: Academy of Finland (AKA)
A novel method was described for producing a layered 3D scaffold consisting of electrospun poly (L,D-lactide) fibers embedded into collagen 1 hydrogel to mimic the natural microenvironment of human pluripotent stem cell (hPSC)-derived neurons. The method enabled the production of a cell-containing scaffold with directed cell growth and neurite extension in a single process. This layered 3D scaffold is a useful model for both in vitro and in vivo neuronal tissue engineering applications.
Background: Three-dimensional (3D) in vitro models have been developed into more in vivo resembling structures. In particular, there is a need for human-based models for neuronal tissue engineering (TE). To produce such a model with organized microenvironment for cells in central nervous system (CNS), a 3D layered scaffold composed of hydrogel and cell guiding fibers has been proposed. New method: Here, we describe a novel method for producing a layered 3D scaffold consisting of electrospun poly (L,D-lactide) fibers embedded into collagen 1 hydrogel to achieve better resemblance of cells' natural microenvironment for human pluripotent stem cell (hPSC)-derived neurons. The scaffold was constructed via a single layer-by-layer process using an electrospinning technique with a unique collector design. Results: The method enabled the production of layered 3D cell-containing scaffold in a single process. HPSCderived neurons were found in all layers of the scaffold and exhibited a typical neuronal phenotype. The guiding fiber layers supported the directed cell growth and extension of the neurites inside the scaffold without additional functionalization. Comparison with existing methods: Previous methods have required several process steps to construct 3D layer-bylayer scaffolds. Conclusions: We introduced a method to produce layered 3D scaffolds to mimic the cell guiding cues in CNS by alternating the soft hydrogel matrix and fibrous guidance cues. The produced scaffold successfully enabled the long-term culture of hPSC-derived neuronal cells. This layered 3D scaffold is a useful model for in vitro and in vivo neuronal TE applications.
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