Characterization and antibacterial activity of Streptomycin Sulfate loaded Bioglass/Chitosan beads for bone tissue engineering

This study aims to the synthesis of a new localized drug delivery system of Bioglass/Chitosan beads as a carrier to an antibiotic drug Streptomycin Sulfate (STRS). In this work, 58S-BG/CH and STRS-loaded 58SBG/CH beads (0% Sm, 10% Sm, 20% Sm, 30% Sm, and 40% Sm) were prepared via the dropwise method. 58S-BG/CH flakes were shaped into beads using Sodium Tri-Polyphosphate (STPP) as a crosslinker. The obtained materials were characterized by different techniques (XRD, FTIR, SEM, and EDXA) before and after in vitro test. The antibacterial activity was investigated against various pathogenic microorganisms such as (Staphylococcus aureus ATCC 29213 and Enterococcus faecalis) as models for Gram-positive Bacteria; (Escherichia coli NCTC 10416 and Klebsiella pneumoniae ATCC 13883) as models for Gram-negative Bacteria using the agar diffusion method. Bioactivity of the prepared beads exhibits a deposition of a layer of hydroxy appetite on their surfaces which confirms the biological activity of them, despite the presence of streptomycin. Also, STRS-loaded BG/CH beads influence the growth of bacteria by appeared of a clearing inhibition zone around the beads dicks compared with BG/CH beads. Published by Elsevier B.V.

Automated summary

This study synthesized a new localized drug delivery system using Bioglass/Chitosan beads as a carrier for the antibiotic Streptomycin Sulfate. The prepared beads exhibited bioactivity with a layer of hydroxy appetite deposition, despite the presence of streptomycin. The STRS-loaded BG/CH beads showed inhibitory effects on bacterial growth compared to BG/CH beads.