期刊
JOURNAL OF MOLECULAR LIQUIDS
卷 325, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.molliq.2020.115148
关键词
Spectroscopic analysis; Vitamin B-12-soy protein isolate; Structure
资金
- National Natural Science Foundation of China [32001686]
- Natural Science Foundation of Heilongjiang Province of China [LH2020C028]
- Heilongjiang Province Applied Technology Research and Development Technology [GA17B002]
- Heilongjiang Province General Undergraduate Higher Education Youth innovative Talents Training Program [NYPYSCT-2018163]
The influence of the interaction between vitamin B-12 and soybean protein isolate (SPI) on the structure of SPI was studied. The results show that as the concentration of vitamin B-12 combined with SPI increases, the structure becomes more orderly. Raman spectroscopy analysis also indicates an increase in interaction between SPI and vitamin B-12 with higher concentration of the vitamin.
In this paper, the influence of the interaction between vitamin B-12 and soybean protein isolate (SPI) on the structure of the SPI was studied by Fourier transform infrared (ITIR), Circular dichroism (CD), and Raman spectra. The results of the FTIR and CD spectra show that when the concentration of vitamin B-12 combined with the SPI increases, the beta-sheet and irregular curl in the SPI will gradually change into an alpha-helix and beta-tum structure, making the structure of SPI more orderly. In addition, the conformation of amide, the vibrational modes of side-chain amino acids and disulfide bonds, and the vibrational modes of aliphatic C-H bonds of SPI were analyzed by Raman spectroscopy. The results showed that the interaction between SPI and vitamin B-12 increased with the increase in the concentration of vitamin B-12. At the same lime, aggregates were formed between the complexes, which made the structure of the SPI tend to be orderly. This paper provides a basis for the study of vitamin B-12 in improving the structure and function of protein. and also provides a theoretical basis for the further study of the interaction between vitamin B-12 and protein. (C) 2020 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据