期刊
JOURNAL OF MEDICAL ENTOMOLOGY
卷 58, 期 5, 页码 1987-1996出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/jme/tjab030
关键词
arbovirus; mosquito; population replacement; antiviral effector; gene-drive
资金
- National Institutes of HealthNational Institute of Allergy and Infectious Diseases (NIH-NIAID) [R01 AI130085-02]
Conventional mosquito control efforts are insufficient in reducing arbovirus prevalence in tropical regions, leading to the development of novel genetic strategies involving genetic manipulation of mosquitoes for population reduction and replacement. Focus is on developing antiviral effectors and gene drives specifically targeting the urban arbovirus vector Aedes aegypti to interrupt arboviral transmission.
Arthropod-borne viruses (arboviruses) such as dengue, Zika, and chikungunya viruses cause morbidity and mortality among human populations living in the tropical regions of the world. Conventional mosquito control efforts based on insecticide treatments and/or the use of bednets and window curtains are currently insufficient to reduce arbovirus prevalence in affected regions. Novel, genetic strategies that are being developed involve the genetic manipulation of mosquitoes for population reduction and population replacement purposes. Population replacement aims at replacing arbovirus-susceptible wild-type mosquitoes in a target region with those that carry a laboratory-engineered antiviral effector to interrupt arboviral transmission in the field. The strategy has been primarily developed for Aedes aegypti (L.), the most important urban arbovirus vector. Antiviral effectors based on long dsRNAs, miRNAs, or ribozymes destroy viral RNA genomes and need to be linked to a robust gene drive to ensure their fixation in the target population. Synthetic gene-drive concepts are based on toxin/antidote, genetic incompatibility, and selfish genetic element principles. The CRISPR/Cas9 gene editing system can be configurated as a homing endonuclease gene (HEG) and HEG-based drives became the preferred choice for mosquitoes. HEGs are highly allele and nucleotide sequence-specific and therefore sensitive to single-nucleotide polymorphisms/resistant allele formation. Current research efforts test new HEG-based gene-drive designs that promise to be less sensitive to resistant allele formation. Safety aspects in conjunction with gene drives are being addressed by developing procedures that would allow a recall or overwriting of gene-drive transgenes once they have been released.
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