期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 69, 期 4, 页码 229-243出版社
SAGE PUBLICATIONS LTD
DOI: 10.1369/0022155421996845
关键词
Bergmann glia; cerebrospinal fluid; gene knockout; glial response; gliocrine; neuroendocrine; X-gal staining
类别
资金
- Japan Society for the Promotion of Science (JSPS) [16K08078, 20K06418, 18K05443]
- joint research program of the Institute for Molecular and Cellular Regulation, Gunma University [18015]
- Nihon University College of Bioresource Sciences Research Grant
- Grants-in-Aid for Scientific Research [20K06418, 18K05443, 16K08078] Funding Source: KAKEN
SgIII is mainly expressed in endocrine tissues such as the pituitary gland, adrenal medulla, and pancreatic islets, with additional expression in the brain and peripheral nervous tissues. The expression of LacZ is particularly prominent in astrocytes.
Secretogranin III (SgIII) is a granin protein involved in secretory granule formation in peptide-hormone-producing endocrine cells. In this study, we analyzed the expression of the LacZ reporter in the SgIII knockout mice produced by gene trapping (SgIII-gtKO) for the purpose of comprehensively clarifying the expression patterns of SgIII at the cell and tissue levels. In the endocrine tissues of SgIII-gtKO mice, LacZ expression was observed in the pituitary gland, adrenal medulla, and pancreatic islets, where SgIII expression has been canonically revealed. LacZ expression was extensively observed in brain regions, especially in the cerebral cortex, hippocampus, hypothalamic nuclei, cerebellum, and spinal cord. In peripheral nervous tissues, LacZ expression was observed in the retina, optic nerve, and trigeminal ganglion. LacZ expression was particularly prominent in astrocytes, in addition to neurons and ependymal cells. In the cerebellum, at least four cell types expressed SgIII under basal conditions. The expression of SgIII in the glioma cell lines C6 and RGC-6 was enhanced by excitatory glutamate treatment. It also became clear that the expression level of SgIII varied among neuron and astrocyte subtypes. These results suggest that SgIII is involved in glial cell function, in addition to neuroendocrine functions, in the nervous system:
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