期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 406, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2020.124434
关键词
Bioluminescence; Biosensing; Enhancement strategies; Graphene-oxide; Mobile-phone; Toxicity assessment
资金
- European Commission Program, H2020-WATER, INTCATCH Project [689341]
- Severo Ochoa Program (MINECO) [SEV-20130295]
- Generalitat de Cataluna [2014 SGR 260]
- EU Graphene Flagship Core 3 Project [881603]
- H2020 Societal Challenges Programme [689341] Funding Source: H2020 Societal Challenges Programme
This study developed a mobile-phone-based sensing platform for water toxicity assessment in just 5 minutes using two common pesticides as model analytes. New methodologies were established to enhance the bioluminescent signal of A. fischeri, and the biocompatibility of graphene oxide to the bacterium was investigated to improve the sensitivity of toxicity assays and bacterial growth.
Recently, many bioluminescence-based applications have arisen in several fields, such as biosensing, bioimaging, molecular biology, and human health diagnosis. Among all bioluminescent organisms, Aliivibrio fischeri (A. fischeri) is a bioluminescent bacterium used to carry out water toxicity assays since the late 1970s. Since then, several commercial A. fischeri-based products have been launched to the market, as these bacteria are considered as a gold standard for water toxicity assessment worldwide. However, the aforementioned commercial products rely on expensive equipment, requiring several reagents and working steps, as well as high-trained personnel to perform the assays and analyze the output data. For these reasons, in this work, we have developed for the first time a mobile-phone-based sensing platform for water toxicity assessment in just 5 min using two widespread pesticides as model analytes. To accomplish this, we have established new methodologies to enhance the bioluminescent signal of A. fischeri based on the bacterial culture in a solid media and/or using graphene oxide. Finally, we have addressed the biocompatibility of graphene oxide to A. fischeri, boosting the sensitivity of the toxicity assays and the bacterial growth of the lyophilized bacterial cultures for more user-friendly storage.
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