期刊
JOURNAL OF ETHNOPHARMACOLOGY
卷 266, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2020.113394
关键词
Qingchang Huashi formula; Ulcerative colitis; Gut microbiota; Goblet cell
资金
- National Natural Science Foundation of China [81930109, 81720108032, 81421005, 91429308, 81973559, 81603193, 81673905]
- Project for Major New Drug Innovation and Development [2018ZX09711001-002-003, 2018ZX09711002-001-004]
- Overseas Expertise Introduction Project for Discipline Innovation [G20582017001]
- Grant National Key R&D Program of China [2017YFC1700104]
- Clinical Medicine Center of Digestive Diseases of Traditional Chinese Medicine in Jiangsu [BL2014100]
- Postgraduate Research and Practice Innovation Program of Jiangsu Province [KYCX19_1197]
The study aimed to define the effect of QHF and its components, Baitouweng (PBR) and Baizhi (ADR) on treating UC. Results showed that QHF significantly inhibited colitis and protected mice from weight loss and colon shortening. ADR and PBR were found to have strong efficacy in inhibiting DSS-induced colitis.
Ethnopharmacological relevance: Inflammatory bowel disease (IBD) is a chronic and relapsing inflammatory disease of the gastrointestinal tract, consisting of ulcerative colitis (UC) and Crohn's disease (CD). Gut microbiota and their metabolites may play a role in the pathogen of IBD, especially of the UC. Qingchang Huashi Formula (QHF), a traditional Chinese medicine formula, has shown therapeutic effect on treating UC based on the clinical practice without clear pharmacological mechanism. Aim of the study: The aim of this study was to clearly define the effect of QHF and its components, Baitouweng (PBR) and Baizhi (ADR) on treating UC. Materials and Methods: Pharmacodynamic effects of QHF and single herb were evaluated in dextran sulfate sodium (DSS) induced acute or chronic colitis mice. Body weight loss, disease activity index (DAI) and colon length were estimated. Histological changes were observed by H&E staining. The number and abundance of gut microbiota were measured with 16S rRNA sequencing. LC-MS and GC-MS were used to detect the concentration of metabolites (e.g., bile acids (BAs) and short chain fatty acids (SCFAs)). The goblet cell was observed by Alcian blue/periodic acid-Schiff (AB/PAS) straining and the crypt stem cell was estimated by immunohistochemical analyses. The colorectal tissues were used to detect levels of IL-1 beta, IL-6 and TNF-alpha by ELISA or qRT-PCR. The expression of NLRP3, Caspase 1 and IL-1 beta were examined by western blotting. Results: QHF significantly inhibited colitis, protected mice from the loss of body weight and colon shorten. Comparatively, ADR and PBR showed strong efficacy in inhibiting DSS-induced colitis. We verified that while ADR was responsible for QHF's effect on maintaining gut microbiota homeostasis and metabolism, PBR was more prominent in keeping crypt stem cells proliferation and colonic goblet cells function. Moreover, we demonstrated that the alleviation of colitis by QHF was associated with the restoration of gut microbiota-metabolism homeostasis, protection of intestinal epithelial barrier and regulation of NLRP3/IL-1 beta pathway. Conclusions: The finding of the present study suggested that QHF is curative in DSS-induced colitis by restoring gut microbiota-metabolism homeostasis and goblet cells function. An optimized QHF was constituted by ADR and PBR, which showed comparable efficacy on colitis to that of QHF. Our work probed out the active constitutes as well as the relevant pharmacological mechanisms of QHF, shedding light on potential new drug combination for the treatment of IBD.
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