Effect of Natural and Artificial Dentin Conditioners on the Release of Vascular Endothelial Growth Factor

Introduction: The purpose of this study was to observe the release of vascular endothelial growth factor (VEGF) after conditioning with etidronic acid (also known as 1hydroxyethylidene-1 and 1-bisphosphonate [HEDP]) and phytic acid (inositol hexakisphosphate [IP6]) in comparison with EDTA on human dentin disks and cylinders. Methods: Human dentin disks were disinfected and prepared by the standardized method. Seventeen percent EDTA, 9% HEDP, 1% IP6, and distilled water (5 minutes) were used to immerse dentin disks. The dentin cylinders were prepared by following disinfection and the standardized preparation method to achieve a truncated cone-shaped canal with a 1-mm open apex. The dentin cylinder samples were irrigated with sodium hypochlorite (5 mL/5 minutes) and then rinsed with 17% EDTA, 9% HEDP, 1% IP6 and distilled water (5 mL/5 min). Enzyme-linked immunosorbent essay was performed to measure VEGF release. One-way analysis of variance was used to compare the mean release of VEGF between study groups and controls at a significance level of .05. A post hoc Tukey test was used for multiple comparisons between study groups. Results: Among conditioners, HEDP released more VEGF from both disks and cylinders. In cylinders, VEGF release by HEDP was significantly greater than the other conditioners, whereas in disks the release of VEGF was similar with all conditioners. Conclusions: The release of VEGF by 9% HEDP and 1% IP6 was comparable with 17% EDTA from dentin disks; however, HEDP demonstrated more release from dentin cylinders than EDTA and IP6. (J Endod 2021;47:800?805.)

Automated summary

This study compared the release of VEGF after conditioning with etidronic acid and phytic acid to EDTA on human dentin disks and cylinders. The results showed that HEDP released more VEGF compared to other conditioners, especially in dentin cylinders.