4.8 Article

Structural tuning of oligonucleotides for enhanced blood circulation properties of unit polyion complexes prepared from two-branched poly (ethylene glycol)-block-poly(L-lysine)

期刊

JOURNAL OF CONTROLLED RELEASE
卷 330, 期 -, 页码 812-820

出版社

ELSEVIER
DOI: 10.1016/j.jconrel.2021.01.001

关键词

Oligonucleotide delivery; siRNA; Antisense oligonucleotide; Unit polyion complex; Polymeric nanocarrier

资金

  1. Center of Innovation (COI) Program of the Japan Science and Technology Agency (JST) [JPMJCE1305]
  2. Project for Cancer Research and Therapeutic Evolution (P-CREATE)
  3. Basic Science and Platform Technology Program for Innovative Biological Medicine (IBIOMED) of the Japan Agency for Medical Research and Development (AMED)
  4. MEXT of Japan (JSPS KAKENHI) [18K18379, 17H02098, 20H00658]
  5. JSPS Core-to-Core Program [JPJSCCS20170007]
  6. Nakatomi Foundation
  7. Noguchi Institute

向作者/读者索取更多资源

The researchers found that chemical modification of oligonucleotides to have higher negative charges facilitated the formation of polyion pairs between the oligonucleotide and bPEG-PLys under harsh biological conditions, leading to enhanced blood circulation of uPICs.
Downsizing nanocarriers is a promising strategy for systemically targeting fibrotic cancers, such as pancreatic cancer, owing to enhanced tissue permeability. We recently developed a small oligonucleotide nanocarrier called a unit polyion complex (uPIC) using a single oligonucleotide molecule and one or two molecule(s) of two-branched poly(ethylene glycol)-b-poly(L-lysine) (bPEG-PLys). The uPIC is a dynamic polyion-pair equilibrated with free bPEG-PLys, and thus, is highly stabilized in the presence of excess amounts of free bPEG-PLys in the bloodstream. However, the dynamic polyion-pairing behavior of uPICs needs to be further investigated for longevity in the bloodstream, especially under lower amounts of free bPEG-PLys. Herein, the polyion-pairing behavior of uPICs was investigated by highlighting oligonucleotide stability and negative charge number. To this end, small interfering RNA (siRNA) and antisense oligonucleotides (ASO) were chemically modified to acquire nuclease resistance, and the ASO was hybridized with complementary RNA (cRNA) to form a hetero-duplex oligonucleotide (HDO) with twice the negative charges. While all oligonucleotides similarly formed sub-20 nm-sized uPICs from a single oligonucleotide molecule, the association number of bPEG-PLys (AN(bPEG-PLys)) in uPICs varied based on the negative charge number of oligonucleotides (N-), that is, AN(bPEG-PLys )= similar to 2 at N- = similar to 40 (i.e., siRNA and HDO) and AN(bPEG-PLys) = similar to 1 at N- = 20 (i.e., ASO), presumably because of the balanced charge neutralization between the oligonucleotide and bPEG-PLys with a positive charge number (N+) of similar to 20. Ultimately, the uPICs prepared from the chemically modified oligonucleotide with higher negative charges showed considerably longer blood retention than those from the control oligonucleotides without chemical modifications or with lower negative charges. The difference in the blood circulation properties of uPICs was more pronounced under lower amounts of free bPEG-PLys. These results demonstrate that the chemical modification and higher negative charge in oligonucleotides facilitated the polyion-pairing between the oligonucleotide and bPEG-PLys under harsh biological conditions, facilitating enhanced blood circulation of uPICs.

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