期刊
JOURNAL OF CONTROLLED RELEASE
卷 330, 期 -, 页码 842-850出版社
ELSEVIER
DOI: 10.1016/j.jconrel.2020.10.057
关键词
Tumor-specific fluorescent dyes; Rhodamine B isothiocyanate; Hemoglobin quenchable fluorescence; Tumor oxidation stress
资金
- National Natural Science Foundation of China [51833008, 21875211, U1501243, 51603181]
- National Key Research and Development Program of China [2016YFA0200301]
- Fundamental Research Funds for the Central Universities of China [2019FZA4020]
Research shows that rhodamine B isothiocyanate derivatives (RBITCs) exhibit unique tumor-specific fluorescence, which can be utilized for cancer imaging and early diagnosis.
Fluorescence is routinely used for in vivo tracking and imaging of molecules and nanostructures with assuming that the fluorescence intensity is proportional to the dye concentration. Herein, we report the unique tumor-specific fluorescence character of rhodamine B isothiocyanate derivatives (RBITCs), which emits fluorescence selectively in cancerous tissues, including small metastatic tumors, but is quenched in blood and healthy tissues. A preliminary mechanism study shows that binding of the thiourea group in the RBITCs on hemoglobin quenches their fluorescence, but the oxidation of the thiourea by the elevated reactive oxygen species in tumor activates the fluorescence. Thus, the fluorescent intensity of RBITCs is associated with the microenvironment of tissues and positively correlates with the cancer stages. These findings suggest that the RBITCs are not suitable for tracking of cargos in the presence of red blood cells but may be useful for cancer imaging and early diagnosis, and probing the tumor microenvironment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据