期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 594, 期 -, 页码 195-203出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2021.02.100
关键词
Sars-CoV-2 nucleoprotein; Diagnostics of COVID-19; SARS-CoV-2; Total internal reflection ellipsometry (TIRE); Surface plasmon resonance; Antigen-antibody complex formation kinetics; Optical biosensors
资金
- European Social Fund [09.3.3LMTK712190106]
- Research Council of Lithuania (LMTLT)
During the pandemic, various methods for SARS-CoV-2 detection and COVID-19 diagnostics were developed, which included antibody and antigen tests. Recent research has shown that the interaction between SCoV2-rN and anti-SCoV2-rN antibodies plays a crucial role in guiding the development of new medications.
During the pandemic, different methods for SARS-CoV-2 detection and COVID-19 diagnostics were developed, including antibody and antigen tests. For a better understanding of the interaction mechanism between SARS-CoV-2 virus proteins and specific antibodies, total internal reflection ellipsometry based evaluation of the interaction between SARS-CoV-2 nucleoprotein (SCoV2-rN) and anti-SCoV2-rN antibodies was performed. Results show that the appropriate mathematical model, which takes into account the formation of an intermediate complex, can be applied for the evaluation of SCoV2-rN/anti-SCoV2-rN complex formation kinetics. The calculated steric factor indicated that SCoV2-rN/anti-SCoV2-rN complex formation has very strict steric requirements. Estimated Gibbs free energy (Delta G(Assoc)) for SCoV-rN and antiSCoV-rN binding was determined as -34 kJ/mol. The reported findings are useful for the design of new analytical systems for the determination of anti-SCoV2-rN antibodies and for the development of new anti-SARS-CoV-2 medications. (C) 2021 Elsevier Inc. All rights reserved.
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