期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 583, 期 -, 页码 704-713出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2020.09.066
关键词
Interfacial displacement; Interfacial rheology; Animal-plant protein mixture; Interfacial structure; Competitive adsorption; Food emulsions; Protein-stabilized emulsions; Oil-water interface
资金
- TiFN, a public -private partnership on precompetitive research in food and nutrition
- Fromageries Bel S.A.
- Nutricia Research B.V.
- Pepsico Inc.
- Unilever Innovation Centre Wageningen BV.
- Netherlands Organisation for Scientific Research
- Topsector AgriFood
- NWO [ALWTF. 2016.001]
This study found that adding a second protein to emulsions can increase surface load, and protein-protein interactions are the key driving force for protein displacement.
Hypothesis: Many traditional or emergent emulsion products contain mixtures of proteins, resulting in complex, non-equilibrated interfacial structures. It is expected that protein displacement at oil-water interfaces depends on the sequence in which proteins are introduced during emulsion preparation, and on its initial interfacial composition. Experiments: We produced emulsions with whey, pea or a whey-pea protein blend and added extra protein post-emulsification. The surface load was measured indirectly via the continuous phase, or directly via the creamed phase. The interfacial composition was monitored over a three-day period using SDSPAGE densitometry. We compared these findings with results obtained using an automated drop tensiometer with bulk-phase exchange to highlight the effect of sequential protein adsorption on interfacial tension and dilatational rheology. Findings: Addition of a second protein increased the surface load; especially pea proteins adsorbed to pre-adsorbed whey proteins, leading to thick interfacial layers. The addition of whey proteins to a pea proteinor whey-pea protein blend-stabilized emulsion led to significant displacement of the pea proteins by 8-lactoglobulin. We determined that protein-protein interactions were the driving force for this displacement, rather than a decrease in interfacial tension. These outcomes could be instrumental in defining new strategies for plant-animal protein hybrid products. (c) 2020 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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