Benign Synthesis of Cobalt Oxide Nanoparticles Containing Red Algae Extract: Antioxidant, Antimicrobial, Anticancer, and Anticoagulant Activity

Eco-friendly synthesis of Cobalt oxide nanoparticles (Co(3)O(4)NPs) was assessed by the aqueous extract of red algae and their utilization as cytotoxicity, antioxidant, anticoagulant, antibacterial, and anti-cancer properties. UV-vis, SEM, TEM, FTIR, EDAX, XRD, and TGA are tools used for characterization purposes. The size of the Co(3)O(4)NPs crystal of 28.2 +/- 7.8 nm was measured based on the X-ray diffraction analysis. Biosynthesized Co(3)O(4)NPs showed uniform spherical morphology with an average diameter of 29.8 +/- 8.6 nm according to TEM and SEM images. Furthermore, Co(3)O(4)NPs biological properties such as determination of the antibacterial properties of Co(3)O(4)NPs by the zone of inhibition method and the minimum inhibitory concentration (MIC) were determined using a dilution broth agar test. Co(3)O(4)NPs indicated higher antibacterial than oxytetracycline standard antibiotics. On the other hand, a scavenging free radical DPPH study was done to determine the propensity for antioxidants that showed a similar capacity for antioxidants in Co(3)O(4)NPs. The biosynthesized Co(3)O(4)NPs are effective erythrocyte viability dose-dependently and indicated this method was nontoxic. Moreover, bioinspired Co(3)O(4)NPs has promising results against HepG2 cancer cells (IC50: 41.4 mu g/ml). Most notably, biogenic Co(3)O(4)NPs have anticoagulant and thrombolytic activities by the addition of nanoparticles to human blood samples under practical conditions. According to these context findings, Co(3)O(4)NPs would be used as a medicinal aid along with its anti-cytotoxic, antioxidant, and effects on bacteria, and cancer.

Automated summary

The eco-friendly synthesis of Cobalt oxide nanoparticles using red algae extract showed potential applications in cytotoxicity, antioxidant, anticoagulant, antibacterial, and anti-cancer properties. The nanoparticles exhibited uniform spherical morphology with significant antibacterial effects and antioxidant capacity. Additionally, they demonstrated effectiveness against cancer cells and had anticoagulant activities in blood samples, making them promising for medicinal use.