期刊
JOURNAL OF CELLULAR PHYSIOLOGY
卷 236, 期 9, 页码 6376-6390出版社
WILEY
DOI: 10.1002/jcp.30312
关键词
macrophage; mechanotransduction; MSC; osteogenesis; YAP; BMP2 axis
资金
- National Natural Science Foundation of China [11532004, 31270990, 31600762]
- Innovation and Attracting Talents Program for College and University (111 Project) [B06023]
This study reveals that macrophages stimulated with mechanical stretch under specific conditions can efficiently induce osteogenic differentiation of MSCs through a synergy mechanism between M2 polarization and YAP/BMP2 axis. Additionally, the mechanical stretch activates macrophages to produce anti-inflammatory cytokines, while also inducing YAP activation and downstream BMP2 expression to facilitate MSCs osteogenesis. These findings advance understanding of the complex influence among mechanical strain, macrophage inflammatory response, and MSC osteogenic differentiation, providing insights into the control system from mechanical signals to chemical response during bone repair and regeneration.
For reconstructive surgeons, critically skeletal damage represents a major challenge. Growing evidence indicate that bone repair is dynamically regulated by the mesenchymal stem cell (MSC)-macrophage interaction. Mechanical strain plays a fundamental role in bone repair and regeneration by influencing MSCs differentiation. Recently, a few findings indicate that macrophages may be mechanically sensitive and their phenotype can be regulated, in part, by mechanical cues. However, how macrophages subjected mechanical stretch influence the osteogenic differentiation of MSCs remain unclear. Thus, the purpose of this study is to explore the effect of macrophages stimulated with mechanical stretch on MSCs osteogenesis. By using a coculture system, we discover that macrophages efficiently induce osteogenic differentiation of MSCs under specific stretch conditions. A synergy mechanism between M2 polarization and YAP/BMP2 axis are identified through molecular and genetic analyses. Macrophages are activated by cyclic stretch and polarized to M2 phenotype that produce anti-inflammatory cytokines such as IL-10 and TGF-beta to regulate the local inflammatory microenvironment. Furthermore, mechanical stretch induces YAP activation and nuclear translocation, subsequently regulates downstream BMP2 expression to facilitate MSCs osteogenesis. These findings not only advance our understanding of the complex influence among the mechanical strain, macrophage inflammatory response as well as the osteogenic differentiation of MSCs, but also reveal a control system from mechanical signals to chemical response then to cell behaviors during bone repair and regeneration.
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