Alkaloids of Adhatoda vasica Nees. as potential inhibitors of cyclooxygenases - an in-silico study

Eicosanoid pathways play a crucial role in the progression and resolution of inflammation. NSAIDs act as anti-inflammatory agents by inhibiting both the isoforms of cyclooxygenases (COXs) whereas, COXIBs act as specific COX-2 inhibitors. Excessive usage of the same is linked with gastrointestinal bleeding and increased cardiovascular risk, respectively. The current in-silico study was aimed at evaluating the potential of major alkaloids of A. vasica (vasicine (VAS), vasicinone (VAE), and Deoxyvasicine (DOV)) as inhibitors of COXs. The results of the computed binding energy (Delta G) indicate that Celecoxib (CEL), DOV, and VAS have a higher affinity to COX-2, while VAE has a higher affinity to COX-1, and Mefenamic acid (MEF) was not selective. Among the alkaloids, VAE exhibited the best Delta G (of -8.2 kcal/mol) with COX-1, while VAS exhibited the best Delta G (of -8.2 kcal/mol) with COX-2. This was comparable to the Delta G exhibited by Mefenamic acid (-8.7 kcal/mol with both the COXs). With their potential to remain gastroprotective while having the ability to inhibit enzymes of both the prostaglandin and leukotriene pathways, the alkaloids of A. vasica could be promising leads for the design of Eicosanoid pathway modulators/inhibitors. Communicated by Ramaswamy H. Sarma

Automated summary

The study evaluated the potential of major alkaloids of A. vasica as inhibitors of COXs, with promising results showing that these alkaloids could be leads for the design of Eicosanoid pathway modulators.