期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 26, 期 3, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.26.3.036002
关键词
Raman spectroscopy; fluorescence guidance; probe tracking; margin delineation; cancer
资金
- NanoMed Marie Sklodowska-Curie ITN from H2020 program [676137]
- H2020 through the Individual Marie Sklodowska-Curie Fellowship IMAGINE [701713]
- EPSRC [EP/L006472/1]
- National Institute for Health Research (NIHR)
- GlaxoSmithKline Engineered Medicines Laboratory
- British Heart Foundation Intermediate Basic Science Research Fellowship [FS/15/33/31608]
- MRC [MR/R026416/1]
- Wellcome Trust
- Wellcome Trust Senior Investigator Award [098411/Z/12/Z]
- National Institute for Health Research
- MRC [MR/R026416/1] Funding Source: UKRI
- Marie Curie Actions (MSCA) [701713] Funding Source: Marie Curie Actions (MSCA)
This study aims to extend Raman spectroscopic diagnostics and develop a multimodal computer vision-based diagnostic system for the detection and precise delineation of tumor margins. The system enables real-time tumor margin delineation and has the potential to improve clinical tumor resection surgeries.
Significance: Tumor detection and margin delineation are essential for successful tumor resection. However, postsurgical positive margin rates remain high for many cancers. Raman spectroscopy has shown promise as a highly accurate clinical spectroscopic diagnostic modality, but its margin delineation capabilities are severely limited by the need for pointwise application. Aim: We aim to extend Raman spectroscopic diagnostics and develop a multimodal computer vision-based diagnostic system capable of both the detection and identification of suspicious lesions and the precise delineation of disease margins. Approach: We first apply visual tracking of a Raman spectroscopic probe to achieve real-time tumor margin delineation. We then combine this system with protoporphyrin IX fluorescence imaging to achieve fluorescence-guided Raman spectroscopic margin delineation. Results: Our system enables real-time Raman spectroscopic tumor margin delineation for both ex vivo human tumor biopsies and an in vivo tumor xenograft mouse model. We then further demonstrate that the addition of protoporphyrin IX fluorescence imaging enables fluorescence-guided Raman spectroscopic margin delineation in a tissue phantom model. Conclusions: Our image-guided Raman spectroscopic probe-tracking system enables tumor margin delineation and is compatible with both white light and fluorescence image guidance, demonstrating the potential for our system to be developed toward clinical tumor resection surgeries. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.
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