4.4 Article

Human epidermal growth factor-functionalized cocoon silk with improved cell proliferation activity for the fabrication of wound dressings

期刊

JOURNAL OF BIOMATERIALS APPLICATIONS
卷 36, 期 4, 页码 722-730

出版社

SAGE PUBLICATIONS LTD
DOI: 10.1177/0885328221997981

关键词

Silkworm; human epidermal growth factor; cocoon silk; transgenic; wound healing

资金

  1. National Natural Science Foundation of China [31772676]

向作者/读者索取更多资源

This study successfully produced transgenic silkworms expressing the hEGF fusion protein using piggyBac transposon technology, and the extracted silk from the cocoons showed a significant increase in cell proliferation without cytotoxicity. The transgenic silk could potentially be a cost-effective and functional biomaterial for wound dressing applications.
Human epidermal growth factor (hEGF) is a key factor involved in wound healing owing to its powerful ability to stimulate cell proliferation. In this study, we used piggyBac transposon technology to produce transgenic silkworms expressing the hEGF protein fused to truncated heavy chain (FibH-hEGF). The FibH-hEGF fusion protein was successfully expressed and secreted into silkworm cocoons. Compared to wild-type silk, the transgenic silkworm silk had the similar morphology about silks fiber surface and cocoon nets, while the secondary structure between the transgenic silk and wild-type silk was different. Most importantly, transgenic silkworm cocoon silk powder extract significantly increased human fibroblast FIB cell proliferation for a long duration with no apparent cytotoxicity. Our study provides a promising method for obtaining cost-effective and functional biomaterials for the fabrication of wound dressings.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据