期刊
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
卷 26, 期 2-3, 页码 283-298出版社
SPRINGER
DOI: 10.1007/s00775-021-01851-1
关键词
Platinum anticancer complex; Iso-pentylglycine; Tert-pentylglycine; MCF-7; DNA interaction; DFT; Molecular docking; ADMET; Circular dichroism; Fluorescence
资金
- Chemistry & Chemical Engineering Research Center of Iran (CCERCI)
- Semnan University
Two new anticancer platinum complexes were synthesized and investigated for their side branches effect on complex-DNA interaction. The tert-pentylglycine ligand exhibited higher DNA binding ability and stronger inhibitory effect on MCF-7 cell line compared to the iso-derivative.
In this study, two new anticancer platinum complexes formulated as [Pt(bpy)(L)]NO3 were synthesized using the iso and tert-pentylglycine ligands, two structural isomer ligands, to investigate side branches effect on the complex-DNA interaction. According to the comparative results of the ADMET assessment, these compounds can be considered as the drug-like molecules and oral medication. Mechanism of tumor inhibition and DNA binding parameters indicated the higher ability of the tert-isomer and also, both complexes acted through the disruption of the base pairs and stacks of helicity by the endothermic process. Fluorescence spectroscopy showed that the quenching mechanism is static for both drugs with large binding constant and high binding affinity towards the DNA. Also, the amount of binding constant of the tert -isomer was about 14 times of another structural isomerous complex. CD spectra indicated the conversion of the B-DNA into A-DNA form via electrostatic interaction for positively charged complexes. The cytotoxic data showed that both compounds have antiproliferative effects against the MCF-7 cell line and the inhibitory effect of the iso-derivative was better than the tert-one. Docking studies showed that the desolvation energy and hydrogen bond are more effective between the other interactions. The torsional free energy for both complexes mainly provided the groove binding along with partially electrostatic and intercalate binding. According to the density-functional theory data and because of positive electron density on the surface of complexes and facilitating of the metal drug to DNA phosphate groups approaching, both complexes may be good candidates for the anticancer drugs. [GRAPHICS] .
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