期刊
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
卷 53, 期 2, 页码 157-167出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10863-021-09886-4
关键词
Phenolic compounds; Antibacterial drugs; Active efflux
资金
- Regional University of Cariri - URCA
- FundacAo Cearense de Apoio ao Desenvolvimento Cientifico e Tecnologico - FUNCAP [BP3-0172-00168.01.00/20 SPU 09673071/2020]
This study evaluated the inhibitory effect of quercetin on S. aureus Efflux Pumps, finding that the inhibition concentration of quercetin was not clinically relevant. A reversal of bacterial resistance effect was observed with strains carrying the TetK and NorA pumps. Furthermore, stability in the interaction between the quercetin complex and the NorA pump was provided by hydrogen bonds formed by the hydroxyl group.
The objective of this work was to evaluate the inhibitory effect of quercetin on S. aureus Efflux Pumps. The MIC of Quercetin was evaluated through the broth microdilution method, as well as the Efflux Pump inhibition assay through the method of reducing the antibiotic minimum inhibitory concentration as well as that of ethidium bromide. The in silico approach through bioinformatics was performed to demonstrate the molecular mechanism of interaction of the substrate and the binding cavity. The Quercetin inhibition concentration was not clinically relevant. With respect to the reversal of bacterial resistance effect by efflux pump inhibition, this effect was observed with the strains carrying the TetK and NorA pumps. Regarding the interaction between the Quercetin complex and the NorA pump, the extra stability was provided by hydrogen bonds produced by the hydroxyl group.
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